Article Sidebar
Published:
Mar 18, 2019Keywords:
Article Details
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.
Main Article Content
Anti-diarrhea effects of polysaccharides from Zingiber officinale rhizome on a rat diarrhea model
Abstract
Purpose: To investigate the anti-diarrhea effects of polysaccharides extracted from the rhizome of Zingiber officinale (ZRP) on diarrhea induced by folium sennae in rats.
Methods: Response surface methodology (RSM) based on the Box–Benhnken design (BBD) was performed to optimize ZRP extraction. Thereafter, experimental diarrheal rats induced by treatment with folium sennae were used to investigate the anti-diarrhea effects of ZRP. Diarrhea index, dilute stool rate, gastric residual rate, and intestinal propulsive rate were investigated. In addition, the levels of brain-gut peptides including cholecystokinin (CCK), ghrelin, and vasoactive intestinal peptide (VIP) in the small intestine of rats with diarrhea were determined using commercial ELISA kits.
Results: Optimal ZRP extraction was obtained with an extraction time of 1.4 h, ratio of water to the raw material of 30 mL/g, and extracting 2 times. ZRP treatment at doses of 50, 100, and 200 mg/kg significantly decreased the dilute stool rate and diarrhea index (p < 0.05) and increased gastric residual rate (p < 0.01) dose-dependently. ZRP lowered intestinal propulsive rate (100 and 200 mg/kg, p < 0.01). All ZRP doses (50, 100 and 200 mg/kg) also significantly reduced the levels of CCK (p < 0.01) but increased the levels of ghrelin and VIP (p < 0.01) in the small intestine.
Conclusion: ZRP exerts significant anti-diarrhea effects on experimental diarrheal rats induced by folium sennae via regulating the levels of brain-gut peptides. Further studies are required to determine if these effects can also be replicated in humans
Keywords: Zingiber officinale, Polysaccharide, Response surface methodology, Anti-diarrhea, Cholecystokinin, Ghrelin, and Vasoactive intestinal peptide
