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Published:
Mar 16, 2019Keywords:
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Main Article Content
Regulatory T cells and anti-inflammatory cytokine profile of mice fed a high-fat diet after single-bulb garlic (Allium sativum L.) oil treatment
Abstract
Methods: The study was conducted with 24 BALB/c male mice divided into six groups consisting of four mice each, i.e., control group (non-HFD, no treatment), HFD group without treatment, HFD administered with simvastatin, and three HFD groups administered SGO doses of 12.5, 25.0 and 50.0 mg/kg, respectively, and continuously treated (with SGO) for 45 days. The relative number of regulatory T cells, IL-10 and TGF-β were measured using flow cytometry.
Results: HFD decreased the expression of regulatory T cells, and the production of IL-10 and TGF-β compared to the control group (p < 0.05). SGO (50 mg/kg) significantly enhanced the activation of regulatory T cells and production of TGF-β in mice fed high-fat diet compared to simvastatin group (p < 0.05). The dose of 25 mg/kg SGO significantly increased the level of IL-10 in mice fed a HFD (p < 0.05).
Conclusion: These results suggest that SGO inhibits inflammatory processes in mice fed a HFD, by enhancing regulatory T cells and the anti-inflammatory cytokine IL-10 and TGF-β. Thus, SGO a promising food supplements and/or therapeutic agent for management of inflammation disorders caused by a HFD.
Keywords: Anti-inflammatory agent, Allium sativum, High-fat diet, Interleukin-10, Regulatory T cells, Transforming Growth Factor-β
