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Published:
May 10, 2018Keywords:
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Main Article Content
Anti-inflammatory activity of Agaricus blazei Murill extract in the spleen of mice fed a high-fat diet
Abstract
Purpose: To examine the effects of A. blazei Murill on the production of proinflammatory cytokine TNF-α and regulatory cytokine IL-10 in the spleen of mice fed a high-fat diet.
Methods: The study was conducted on 25 BALB/c male mice divided into five groups consisting of five mice in each group and utilized three A. blazei Murill extract doses: 100, 200 and 400 mg/kg. The mice were fed a high-fat diet (HFD) and given A. blazei Murill extract for 12 weeks. The relative number of CD4+TNF-α+, CD11b/c+TNF-α+, and CD4+CD25+IL-10+ were measured using flow cytometry.
Results: Oral administration of A. blazei Murill extract (100 mg/kg) in mice fed a high-fat diet significantly decreased (p < 0.05) the level of TNF-α that produced by CD4+ T cells and macrophages (1.64 %), compared with control. The 200 mg/kg dose decreased the level of CD11b/c+TNF-α+ by 5.37 % (p < 0.05) compared to 100 mg/kg dose. The 400 mg/kg dose significantly enhanced regulatory cytokines IL-10 by 17.56 % (p < 0.05) in mice fed a high-fat diet.
Conclusion: This findings suggest that Agaricus blazei Murill can inhibits processes in HFD-induced mice by reducing TNF-α production and increasing the anti-inflammatory cytokine IL-10. These results provide new insight into the pharmacological actions of Agaricus blazei Murill as a medicinal food for potential therapy of atherosclerosis disease.
Keywords: Anti-inflammatory agent, Agaricus blazei Murill, Proinflammatory cytokine, TNF-α, IL-10
