Article Sidebar
Published:
Mar 4, 2016DOI:
10.4314/tjpr.v15i2.1Keywords:
Article Details
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.
Main Article Content
Formulation of a New Generation of Liposomes from Bacterial and Archeal Lipids
Abstract
Purpose: To evaluate the application of bacterial liposomes and archaeosomes as a novel drug delivery system for in vitro cytoplasmic delivery of molecules into cancer cells.
Methods: Bacterial membrane lipids were extracted using chloroform and methanol. Bacterial liposomes and archaeosomes of E. coli, Acidianus brierleyi and Sulfolobus acidocaldarius were prepared using film method and their trailing in cancer cells (HT-29) was evaluated by carboxyfluorescein (CF). Their morphological characteristics were assessed by atomic force microscopy (AFM).
Results: At 37 °C, the liposomes and archaeosomes interacted with cell membranes predominantly by fusion and endocytosis. The AFM images showed uniform and dispersed distribution of the liposomes.
Conclusion: The findings demonstrate that bacterial liposomes and archaeosomes may be useful as drug delivery carriers for the treatment of cancer.
Keywords: Liposome, Archaeosome, E. coli, Acidianus brierleyi, Sulfolobus acidocaldarius, Cancer, HT-29 cell, Atomic force microscopy, Film method
