Article Sidebar
Published:
Oct 12, 2015Keywords:
Article Details
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.
Main Article Content
Charge-Transfer Reaction of Cediranib with 2,3-Dichloro- 3,5-dicyano-1,4-benzoquinone: Spectrophotometric Investigation and Use in Development of Microwell Assay for Cediranib
Abstract
Purpose: To investigate the charge-transfer (CT) reaction between cediranib (CRB) and 2, 3 - dichloro-5, 6- dicyano -1, 4 - benzoquinone (DDQ) and employment of the reaction as a basis for the development of a novel 96 - microwell spectrophotometric assay for CRB.
Method: The reaction of CRB and DDQ was investigated in different solvents of varying dielectric constant and polarity index The reaction resulted in the formation of a red-colored product. Spectrophotometric investigations confirmed that the reaction proceeded through to the formation of a (CT) complex.
Results: The molar absorptivity of the CT complex was linearly correlated with the dielectric constant and polarity index of the solvent; the correlation coefficients were 0.801 and 0.858, respectively. The association constant of the complex was 0.5 × 103 L mol−1 in 2-propanol. The reaction conditions were optimized for the development of the microwell assay for CRB. The assay limits of detection and quantitation were 6.8 and 20.6 μg/well, respectively. The assay was validated as per the guidelines of the International Conference on Harmonization (ICH) and successfully applied to the analysis of CRB in its bulk and dosage forms with good accuracy and precision.
Conclusion: The developed assay has high throughput and consumed minimum volume of organic solvent; thus, it reduces the exposure of the analysts to the toxic effects of organic solvents, and should significantly lower the analysis cost.
Keywords: Cediranib, 2,3-dichloro-3,5-dicyano-1,4-benzoquinone, Charge-transfer reaction, Spectrophotometry, Microwell assay, High throughput analysis.
