Article Sidebar
Keywords:
Article Details
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.
Main Article Content
Evaluation of Antioxidant and Anti-neuroinflammatory Activities of Hizikia fusiformis (Harvey) Okamura Extract
Abstract
Purpose: To evaluate the in vitro antioxidant and anti-neuroinflammatory activities of Hizikia fusiformis (Harvey) Okamura extract (HFE) in lipopolysaccharide (LPS)-stimulated murine BV-2 microglial cells.
Methods: The antioxidant activity of HFE was evaluated by measuring 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical-scavenging activity using an ESR spectrometer. Cell viability was estimated by 3-(4, 5- dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. LPS-stimulated BV-2 microglia were used to study the expression and production of inflammatory mediators such as nitric oxide (NO), inducible NO synthase (iNOS) and Interleukin 6 (IL-6).
Results: Treatment with Hizikia fusiformis extract (HFE) significantly scavenged the DPPH radicals with half-maximal concentration of (IC50) of 9.64 ± 0.78 μg/ml (p < 0.01 at 10 μg/ml). The increased levels of NO (35.32 ± 3.62 μM) and protein expressions of iNOS were inhibited by HFE extract in LPS-stimulated BV-2 cells. Increased pro-inflammatory cytokines such as TNF-α and IL-6, were also significantly suppressed by HFE (p < 0.001 at 80 μg/ml). Further, HFE blocked the expression of NF-κB activation in LPS-stimulated BV-2 cells.
Conclusion: HFE can be considered as a useful therapeutic and preventive approach for the treatment of neurodegenerative diseases and oxidative stress-related diseases.
Keywords: Hizikia fusiforme, Antioxidant activity, Anti-neuroinflammation activity, Inducible nitric oxide synthase, Interleukin-6
