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Effect of Carvacrol on Salmonella Saintpaul Biofilms on Stainless Steel Surface
Abstract
Purpose: To evaluate the effect of carvacrol against Salmonella saintpaul biofilms on stainless steel surface.
Methods: The effect of carvacrol on planktonic cells of S saintpaul was evaluated by determining the minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). The action of carvacrol on S. saintpaul biofilms on stainless steel surface was evaluated on established biofilm and on biofilm formation by counting the number of bacterial cells that adhered to the surface and by scanning electron microscopy.
Results: The antimicrobial activity of carvacrol against planktonic cells of S. saintpaul was observed. The highest inhibitory effect of carvacrol was observed during biofilm formation at 78 and 117 µg ml-1 when S. saintpaul biofilms were reduced by 3 log cycles (p < 0.05).
Conclusion: Carvacrol reduced the number of bacterial cells that adhered to stainless steel surface, making it a pot Salential compound for Salmonella saintpaul control.
Keywords:Salmonella spp., Biofilm, Carvacrol, Antimicrobial activity, Planktonic cells, Stainless steel
