Article Sidebar
Published:
Dec 10, 2014Keywords:
Article Details
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.
Main Article Content
Online monitoring for Strychnos nux-vomica parching in sands and chemical compositional analysis by ultra performance liquid chromatography - linear trap quadrupole -orbitrap- mass spectrometry
Abstract
Purpose: To provide an online and non-contact method to monitor the dynamic temperature of the nuxvomica parching in sands, and also to develop a rapid screening and comparison of the compounds in crude and processed nux-vomica.
Methods: Seeds of nux-vomica were parched in sands by an herbal medicine roaster with an onlinetype and non-contact temperature measurement system (ONTMS). Processing was monitored by ONTMS and the processing temperature was accurately controlled within the range 200 - 220 oC. Ultra performance liquid chromatography - linear trap quadrupole -orbitrap-mass spectrometry method in positive ionization mode was established in this work. The samples were extracted by ultrasonication with 50 % methanol for 30 min. Chromatographic separation was achieved on an Acquity UPLC HSS T3 column using a gradient program with acetonitrile and 1 % formic acid as the mobile phase and a diode array detector set at 254 nm for quantification.
Results: The surface color of the processed seeds of nux-vomica were brown or dark brown and the cross-section bulged with small bubble which meets the requirements of Chinese traditional processing methods. Fourteen major compounds from crude and processed nux-vomica were identified simultaneously by HPLC-DAD. According to the MS fragmentation pathways and by comparing with reference literature, the structures of eleven of the compounds were confirmed.
Conclusion: Methods using ONTMS are more accurate for parching nux-vomica in sands and UPLCLTQ- Orbitrap-MS is a rapid and straightforward method for compound profiling in crude and processed nux-vomica.
Keywords: Strychnos, Nux vomica, Parching in sands, Online-type and non-contact temperature measurement system , Ultra performance liquid chromatography - linear trap quadrupole - orbitrap - mass spectrometry , Compound profiling
