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Published:
Dec 10, 2014Keywords:
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Antinociceptive properties and acute toxicity of ethanol extract of Bromelia laciniosa Mart. ex Schult. f. (Bromeliaceae)
Abstract
Purpose: To investigate the antinociceptive activity and acute toxicity of the ethanol extract of Bromelia laciniosa leaf.
Methods: A high performance liquid chromatography HPLC fingerprint of phenolic compounds was developed. The antinociceptive effect of ethanol extract (Bl-EtOH) in mice was carried out using chemical (writhing and formalin) and thermal (hot plate) models of nociception. The acute toxicity of the extract was performed in mice using doses of 2.0 g/kg intraperitoneally and 5.0 g/kg orally. Blood was removed for laboratory analysis of hematological and biochemical parameters.
Results: Bl-EtOH (100, 200 and 400 mg/kg, i.p.) reduced the number of writhing (91.80, 93.44 and 78.68 %, respectively) and the number of paw licks during the first (60.86, 62.84 and 66.79 %) and second phase (91.93, 82.18 and 88.73 %) of the formalin test. Naloxone (1.5 mg/kg, i.p.) antagonized the antinociceptive action of Bl-EtOH (100 mg/kg), and this finding suggests involvement of opioid mechanism. The effect of Bl-EtOH on hot plate response provides a confirmation of its central effect.
Conclusion: B. laciniosa leaf extract has antinociceptive properties. Peripheral, and at least in part, central mechanisms, may be involved in this antinociceptive effect. The ethanol leaf extract apparently presents no significant toxicity.
Keywords: Bromelia laciniosa, Nociception, Pain, Writhing, Acute toxicity
