Article Sidebar
Article Details
Copyright is owned by the Institution of the Agricultural Research and Higher Education (IRESA), Tunisia.
Online open access to full texts of Tunisian Journal of Plant Protection (TJPP) is with no subscription fees. The objective of publishing TJPP in open access is to freely disseminate research results for plant health specialized scientists in order to globally exchange and generalize knowledge in this field.
All TJPP publications are available as Attribution/Non-Commercial works under the Creative Commons License (CC BY-NC) which means that TJPP contents may be freely copied and distributed only for non-commercial purposes providing that credit is given to the publisher and the authors.
Main Article Content
Prevalence and Genetic Diversity of Grapevine Virus D in Tunisia
Abstract
The prevalence and the genetic diversity of grapevine virus D (GVD) isolates from rootstocks, wine and table grape varieties grown in Tunisia were studied. RT-PCR assays performed on the coat protein gene (CP) showed the presence of GVD in 31.5% of the 403 samples tested. The highest rate of infection was found in table grapes (56.5%), followed by autochthonous table grapes (24.1%), wine grapes (20.8%) and rootstocks (12.5%). Sequences and phylogenetic analyses of the partial CP genes of 14 GVD isolates showed nucleotide identities that ranged from 84% to 99%. The Tunisian GVD-isolates segregated in 3 phylogenetic groups together with international isolates reported in GenBank. The present study extends our knowledge of the presence of GVD in Tunisian vines and on its genetic diversity, which is useful for developing broad-spectrum molecular diagnostics (RT-PCR) capable of detecting the different isolates infecting vines.
