Vitamin E or Red Palm Oil Increases The Level Of Nitric Oxide In Wistar Rats Chronically Exposed To Dichlorvos

Abstract

Plasma Nitric oxide concentration was determined in 50 wistar rats exposed to 2,2dichlorovinyl dimethyl phosphate (DDVP) by inhalation. The animals were divided into ten groups of 5 rats per group.The groups were as follows; one unexposed group, six groups exposed to DDVP for 1 week, 2 weeks,3 weeks,4 weeks,5 weeks and 6 weeks in a poorly ventilated compartment, one group was administered with vitamin C, another group was administered with vitamin E and the last group administered with red palm oil. DDVP was prepared in a dilution of 1:1 as recommended by manufacturer for domestic use. Exposure to DDVP was done for 4hours daily. At completion of exposure, animals were anaesthetized and plasma separated from blood obtained by cardiac puncture was used for measurement of Nitric Oxide (NO) concentration using Griess reagent.

Results showed a significant decrease in plasma NO concentration of rats exposed to DDVP for 5weeks when compared with unexposed rats, rats exposed for 1 week, 2 weeks, 3 weeks, and 4 weeks. There were significant increases in plasma NO concentrations of Vit E or RPO supplemented unexposed rats when compared with unsupplemented rats exposed for 5 weeks.

It could be concluded from this study that longtime DDVP inhalation reduces NO level and that vit E or RPO increases the level of NO in rats chronically exposed to DDVP.

Keywords: Plasma Nitric oxide, 2,2 dichlorovinyl dimethyl phosphate(DDVP), inhalation, Wistar rats, vitamin E, Red Palm oil, Griess reagent