Pattern of haemopoietic progenitor cells differentiation and differentiation enhancing effect of Telfairia occidentalis extract in irradiated guinea-pig

Abstract

Ionizing radiation is essentially considered a viable treatment modality for human malignancies. Acute and chronic toxicities with severe morbidity and mortality are well documented. Experimental and clinical approaches to correcting radiation haemopoietic syndrome deficiencies have focused on cytokine activity with same documented toxicities. Limited data is available on differentiation enhancing effects of plant extracts on haemopoietic progenitor cells following radiation injury. This study aimed to determine the potential haemopoietic progenitor cells differentiation effects of Telfairia occidentalis in irradiated guinea pigs. Bone marrow cells from irradiated guinea pigs were harvested and treated with varying concentrations of 0.313-100% of the extracts of the Telfairia occidentalis and the degree of differentiation of the cultured guinea-pig bone marrow haemopoietic stem cells determined. The concentration of the extract ranges from 0.313-100%. This showed Mean ± SD of 3.54±0.30, 0.18±0.01 and 0.88±0.05 for the extract treated, non-extract treated (-ve control) and GM-CSF (+ve control) treated culture plates respectively [p=0.000]. Extract concentrations corrected positively with differentiation of the cultured guinea-pig bone marrow haemopoietic stem cells at 'r' 0.835 [r= 0.812; p=0.001]. Conclusively, Telfairia occidentalis extract has positive differentiation enhancing effects on haemopoietic progenitor cells with deflection in favour of erythroid lineage.