Serum Creatinine and Urea Concentrations of Wistar Rats Exposed to Glacial Acetic Acid

Abstract

This study investigated the effect of exposure to different concentrations of glacial acetic acid (GAA) on serum urea and creatinine concentrations of  wistar rats. Wistar rats were grouped into 5: based on concentrations of  exposure and gender. At the end of four weeks of exposure period,  animals were sacrificed and 5ml of blood collected for urea and creatinine estimation.

Result showed average weight increase of rats from 162±25.19g before  exposure to 204±37.82g after exposure to GAA. The mean±standard  deviation of serum urea and creatinine concentrations of wistar rats  exposed to GAA were; 66.6±10mg/dl and 41.0±7.3 mmol/l while that of  the unexposed group were 63.0±38.5mg/dl and 36.1±2.3 mmol/l  respectively with a P-  value of p>0.05. The mean serum urea concentration of exposed rats to different concentrations of GAA are: 66.7±10.1 mg/dl, 64.2±8.2 mg/dl, 70.0±5.1 mg/dl and 64.6±10.2 mg/dl  for 2%, 5%, 10% and 15% respectively and that of  unexposed was 62.1±8.4 mg/dl with P- value of ( p>0.05) for  2%, 5%, and 15% and a significant p- value of (p<0.05) for 10% when exposed were compared with unexposed. The mean serum creatinine concentrations of exposed rats to different  concentrations of GAA were: 41.0±7.3 mmol/l, 37.7±4.2 umol/l,  40.0±3.8umol/l and 37.0±3.5umol/l for 2%, 5%, 10% and 15%  respectively and that of unexposed was 36.1±2.3umol/l with a non-significant P- value of (p>0.05) for 2%, 5%, and 15% and a significant p-value of (p<0.05) for 10%. This study suggests that inhalation of GAA may significantly affect serum concentrations of urea and creatinine, it is therefore important for safety rules to be in place for safe handling of GAA and other chemicals.

Keywords: Glacial acetic acid, inhalation, urea, creatinine.