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Published:
Apr 15, 2019Keywords:
Conservation Genetic Diversity RAPD markers Terminalia arjuna (Arjun)
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Genetic variation, structure and conservation status of Terminalia arjuna L. (Arjun) in Achanakmar Amarkantak Biosphere Reserve (AABR), Central India
Abstract
Terminalia arjuna has received much
attention in the recent years by its
remarkable value in medicinal, sericulture
and tannin industry, with no prior reported
genetic characterization studies.Sixteen
genotypes of T. arjuna with diverse
geographical locations from three natural
populations (Achanakmar, Chhaparwa and
Lamini) of AABR were selected for this
study. Ten Random Amplified Polymorphic
DNA (RAPD) markers were used to
evaluate the genetic diversity of T. arjuna.
Among ten RAPD decamer primers only six
primers produced the clear and scorable
bands. Six RAPD primer combinations
generated a total of 515 bands of which 213
bands were polymorphic with 41.34±6.23
polymorphic percentage. The average
genetic diversity and polymorphic
information content (PIC) of T. arjuna in
AABR was recorded as 0.193±0.018 and
0.323±0.01 respectively. The dendrogram
constructed through UPGMA algorithm
based on the binary matrix classified all the
16 genotypes into three major clusters; A, B
and C. The population of Lamini was found
genetically more diverse (0.263 ± 0.033) and
the population of Achanakmar was found
less genetically diverse (0.157±0.030). The
fixation index values (F) indicated more
number of heterozygotes in Lamini
population of AABR. The AMOVA
confirmed that 8% of the genetic diversity
exists between the populations and 92% of
the genetic diversity was recorded within the
T. arjuna populations of AABR. This study
provides a new insight into the exploitation
of genetically diverse populations of
Terminalia arjuna from AABR, as a
potential resource for future tree breeding
programmes
attention in the recent years by its
remarkable value in medicinal, sericulture
and tannin industry, with no prior reported
genetic characterization studies.Sixteen
genotypes of T. arjuna with diverse
geographical locations from three natural
populations (Achanakmar, Chhaparwa and
Lamini) of AABR were selected for this
study. Ten Random Amplified Polymorphic
DNA (RAPD) markers were used to
evaluate the genetic diversity of T. arjuna.
Among ten RAPD decamer primers only six
primers produced the clear and scorable
bands. Six RAPD primer combinations
generated a total of 515 bands of which 213
bands were polymorphic with 41.34±6.23
polymorphic percentage. The average
genetic diversity and polymorphic
information content (PIC) of T. arjuna in
AABR was recorded as 0.193±0.018 and
0.323±0.01 respectively. The dendrogram
constructed through UPGMA algorithm
based on the binary matrix classified all the
16 genotypes into three major clusters; A, B
and C. The population of Lamini was found
genetically more diverse (0.263 ± 0.033) and
the population of Achanakmar was found
less genetically diverse (0.157±0.030). The
fixation index values (F) indicated more
number of heterozygotes in Lamini
population of AABR. The AMOVA
confirmed that 8% of the genetic diversity
exists between the populations and 92% of
the genetic diversity was recorded within the
T. arjuna populations of AABR. This study
provides a new insight into the exploitation
of genetically diverse populations of
Terminalia arjuna from AABR, as a
potential resource for future tree breeding
programmes
