Influence of Lindane or Fenitrothion on the response to treatment of trypanosomosis in rats

Abstract

Studies were conducted on the influence of the insecticides, Undone or fenitrothion on the course of treatment of experimental trypanosomosis infection in rats by evaluating changes in hematological and biochemical parameters : n groups of animals. Groups of rats were pretreated with either lindane or fenitrothion, infected with Trypanosoma brucei, before treatment with Berenil 7or quinapyramine.

It was observed that the group of animals pretreated with fenitrothion before administration of Berenil 7 had significantly (P<0.05) higher packed cell volume(PCV), hemoglobin concentration (Hb) total white blood cell count (WBC) and red blood cell count (RBC) when compared to the untreated control and those treated with berenil alone. The hematological parameters from the group of rats pretreated with fenitrothion before the use quinapyramine, were not significantly (P>0.05) different from those treated with quinapyramine alone though the group given quinapyramine alone showed slightly higher serum biochemical parameters, Na⁺, Cl^-, HCO₃², alkaline phosphatase ALP), alanine aminotransferase(ALT), aspartate aminotransferase (AST), and serum total protein when compared to the pretreated group. This may suggest the influence of fenitrothion on the metabolism of quinapyramine.

Animals pretreated with lindane before treating with berenil had lower PCV, and Hb compared to the untreated control and may suggest that lindane had induced the metabolism of berenil.This observation is corroborated by the higher PCV, Hb, and RBC values of the group treated with berenil alone. Furthermore, the observation that the group pretreated with lindane before administering berenil had lower ALT, and AST compared to the untreated control group shows that the berenil had started action to reduce the parasitaemia and therefore tissue damage, before its metabolism.

The group pretreated with lindane before giving quinapyramine, had significantly (P<0.05) lower AST, ALT, and serum protein levels compared with the untreated controls. This may suggest the non influence of lindane on
quinapyramine metabolism.

Key words: Trypanosomosis, insecticides, berenil, quinapyramine, haematology, serum biochemistry.