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Erasto, P., & Mbwambo, Z. (2009). Antioxidant activity and HPTLC profile of Lagenaria siceraria fruits. Tanzania Journal of Health Research, 11(2). https://doi.org/10.4314/thrb.v11i2.45206
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Antioxidant activity and HPTLC profile of Lagenaria siceraria fruits
Abstract
Abstract: The fruits of Lagenaria siceraria Standl. (Cucurbitaceae) are widely used for medicinal and nutritional purposes in Africa. The health promoting ability of the fruits might be related to antioxidant properties of
its constituents. In this study the antioxidant effect of fresh and dried fruits of L. siceraria was evaluated by comparing the 2,2-diphenyl-1,1-picrylhydrazyl (DPPH) radical scavenging and reducing capacity of ethyl
acetate and n-butanol extracts of fresh and dried fruits. The comparison was further emphasized by high performance thin layer chromatography (HPTLC) analysis of the extracts so as to relate activities with their
chemical profiles. Results indicated that ethyl acetate (EA) extract of the fresh fruits exhibited higher DPPH radical scavenging activity than other samples. At 0.01 mg/ml the order of activity was: EA dried fruits (50.6%)
< Bt (n-butanol) fresh fruits (53.3%) < Bt (n-butanol) dried fruits (64.8%) < EA fresh fruits (68.6%) < Gallic acid (81.8%). A slight change of activity was observed at 0.1 mg/ml, where the order was; EA dried fruits (70%) <
Bt dried fruits (71.8%) ≤ Bt fresh fruits (72%) < EA fresh fruits (81.6%) < Gallic acid (88.5%). In the reducing capacity assay, Bt fresh fruits extract exhibited higher reducing power than all test samples. The HPTLC chemical profiles of both fresh and dried fruit extracts showed some slight differences. The slight differences in antioxidant activities were justified by the HPTLC chemical profiles of the fruits. Therefore, taking fresh or
dried fruits of L. siceraria may relatively give similar antioxidant effects. Since the fruits of this plant matures in bulky, then drying, milling and packing the products under hygiene environment can ensure a constant
supply of antioxidant supplement.
its constituents. In this study the antioxidant effect of fresh and dried fruits of L. siceraria was evaluated by comparing the 2,2-diphenyl-1,1-picrylhydrazyl (DPPH) radical scavenging and reducing capacity of ethyl
acetate and n-butanol extracts of fresh and dried fruits. The comparison was further emphasized by high performance thin layer chromatography (HPTLC) analysis of the extracts so as to relate activities with their
chemical profiles. Results indicated that ethyl acetate (EA) extract of the fresh fruits exhibited higher DPPH radical scavenging activity than other samples. At 0.01 mg/ml the order of activity was: EA dried fruits (50.6%)
< Bt (n-butanol) fresh fruits (53.3%) < Bt (n-butanol) dried fruits (64.8%) < EA fresh fruits (68.6%) < Gallic acid (81.8%). A slight change of activity was observed at 0.1 mg/ml, where the order was; EA dried fruits (70%) <
Bt dried fruits (71.8%) ≤ Bt fresh fruits (72%) < EA fresh fruits (81.6%) < Gallic acid (88.5%). In the reducing capacity assay, Bt fresh fruits extract exhibited higher reducing power than all test samples. The HPTLC chemical profiles of both fresh and dried fruit extracts showed some slight differences. The slight differences in antioxidant activities were justified by the HPTLC chemical profiles of the fruits. Therefore, taking fresh or
dried fruits of L. siceraria may relatively give similar antioxidant effects. Since the fruits of this plant matures in bulky, then drying, milling and packing the products under hygiene environment can ensure a constant
supply of antioxidant supplement.
