Assessment of Pancreatic and Hepatic Histological Changes in Streptozotocin Induced Diabetic Rats Fed with Morizella Juice

Abstract

Background: Hibiscus sabdariffa and Moringa oleifera has been reported to have ant diabetic effect on hyperglycemic animals. However, there are no reports on its histological and physiological changes in diabetic animals.

Objective: To assess the pancreatic and hepatic histological changes in streptozotocin-induced diabetic rats fed with Morizella juice.

Methods: A Completely Randomized Block Design (CRBD) was used for this study, 40 adult male Wistar rats, 9 to 10 weeks with a bodyweight 190g – 250g were selected for the study. The animals were divided into four main groups (A, B, C, and D), each group was further subdivided into two groups with 5 rats. The experiments were divided into acute and sub-acute tests of 10 animals per group. At the end of the experiment, animals were sacrificed, pancreatic and liver tissues were harvested and histologically processed. Sections were examined and photographed using a light microscope (Leica® DM 750) with an in-built camera ((Icc50 HD-47142065) and the number of Islet cells was measured in 40 high-power fields.

Results: Microscopic structures of the pancreas revealed that the number of Islets of Langerhans was significantly higher in the Morizella treated groups at the dosages of 50mg/kg and 100 mg/kg body weight. The microscopic structure of the liver in both treated groups presented with normal histological architecture when compared with both control groups

Conclusion: There was a dose-dependent increase of islets of Langerhans cells in the acute and sub-acute group treated with Morizella extracts compared to the control group. Additionally, the liver architecture was reversed back to the normal in Morizella-treated group.