In vitro study on inhibition of glycosylation of methanolic leaf extract of Hibiscus cannabinus

Abstract

The inhibitory properties of Methanolic leaf extract of Hibiscus cannabinus (Malvaceae family) on glycosylation formation, was investigated in haemoglobin using Gallic acid as Standard. The periodic glycosylation of haemoglobin at varying concentration of glucose shows a decrease in haemoglobin concentration indicating the glycosylation of haemoglobin. While the subsequent administration of Hibiscus cannabinus Methanolic leaf extract inhibit haemoglobin glycosylation, where a concentration of 20 mg/ml of the
extract gave a significant inhibition by yielding haemoglobin concentration of 1.877±0.40 ìg/ml for test extract as against 0.032±0.013 ìg/ml for the standard. This suggests that the plant extract inhibits the binding of glucose to hemoglobin, since at higher concentration of glucose the concentration was found to be high.