Antimicrobial Activity of Silver Nanoparticles Synthesized by Marine Lactobacillus Sp against Multiple Drug Resistance Pathogens

Abstract

In this work, in vitro biosynthesis of silver nanoparticles was achieved using AgNO3 as a substrate by L. plantrum isolated from mangrove rhizosphere region in South East Coast of India (Gulf of Mannar). The biosynthesis was faster within a minute of silver ion coming in contact with the cell filtrate. Presence of silver nanoparticles in the culture filtrate was confirmed by absorption peak at 430 nm. The biosynthesis of nanoparticles was the maximum when the culture filtrate was treated with 1.0mM AgNO3 and pH 6.0, incubated at 5 .C for 24 h and its nanoparticles was used for antimicrobial activity. The culture filtrate, precipitated with ammonium sulphate, was proved to have a single protein band with a molecular weight of 70 kDa using polyacrylamide gel electrophoresis. The present work highlighted the possibility of using the marine bacterial strain of L. plantrum to achieve a fast rate of nanoparticles synthesis and also used as antimicrobial agent.