Cytotoxic Activity of Croton gibsonianus Nimm. Grah

Abstract

The present study was designed to investigate the cytotoxic activity of
methanol extract of Croton gibsonianus Nimm. Grah (Euphorbiaceae) leaves
by brine shrimp lethality bioassay. The powdered leaf material was extracted
with methanol. The cytotoxicity activity of methanol extract was tested using
brine shrimp (Artemia nauplii) lethality assay. The mortality of shrimps were
determined and LC50 was calculated. Cytotoxic activity, in terms of mortality
of brine shrimps, was dependent on concentration of the extract. Highest
mortality (87%) was observed at extract concentration 1000ìg/ml. The LC50
of methanol extract was found to be 109.48ìg/ml. The result of the study
indicated that the extract possesses cytotoxic property. Further studies on
isolation of active constituents possessing cytotoxic activity their cytotoxicity
on various cancer and tumor cell lines are under investigation.