Disinfectant effects of Purit®, Z-Germicide® and Carcil® on bacterial isolates from hatcheries in Kaduna state, Nigeria

Abstract

Hatcheries in Kaduna State, Nigeria were investigated for isolation of aerobic bacteria. The following were isolated: Escherichia coli, Proteus sp, Pseudomonas sp, Staphylococcus aureus, Staphylococcus sp and Micrococcus sp. On these isolates, the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and kill-time of two commonly used and one relatively new disinfectant were determined. The two commonly used disinfectants were Purit® (chlorhexidine gluconate Bp 0.3 % w/v and cetrimide Bp 3.0 % w/v) and Z-germicide® (7 % tar acid phenol and 2 % cresol). The new one was Carcil® (Alkyl-benzyl-dimethyl ammonium chloride). It was observed that Purit® was most effective against E.coli since it required the lowest concentration of 0.00026 % for 1 minute to kill the bacteria. Z-germicide® was found to be effective at 0.00063 % for 5 minutes and Carcil® required 0.0031 % for 10 seconds. Purit® was also found to be the most effective of the three disinfectants on Pseudomonas sp but Z-germicide® showed best results on Proteus sp.