Evaluation of antibody response by dogs vaccinated with low egg passage, flurry strain anti-rabies vaccine using single and multiple sites

Abstract

Twelve dogs were immunized with live attenuated flury strain antirabies vaccine using single, double and triple inoculation sites of the recommended dose, but in divided doses for the double and triple sites. All the dogs were screened for neutralizing antibodies against rabies before immunization, while sera were collected on day 1, 3, 7, 10, 14, and 28 post immunization. These sera were analyzed for viral neutralization and protection using mouse neutralization test (MNT). All the sites induced sero-conversion in all subjects from day one post vaccination, but the levels of neutralizing antibody production with triple site regime showed a four-fold increase in serum titre over the two-site regime. A 100% sero-conversion was achieved by day 28 post vaccination for the triple-site regime.