Comparative evaluation of serum amyloid A concentration in Red Sokoto bucks neutered using different procedures

Abstract

In this study, the changes in concentrations of serum amyloid A (SAA) induced by orchidectomy, Burdizzo castration, and in-situ spermatic cord ligation in Red Sokoto bucks were evaluated. Sixteen (16) Red Sokoto bucks, 6 months to one year old and weighing between 11kg and 12 kg, were randomly divided into 4 groups (A-D), each comprising 4 bucks. Bucks in group A were castrated using the Burdizzo method, B in-situ spermatic cord ligation, and C orchidectomy while group D served as control. Blood was collected from each buck, serum was harvested and analyzed for SAA at 0, 4, 8, 12, 15, 20, 24, 36, 48 and 72 hours post-castration. Results revealed significant increase (p=0.028) in the levels of SAA in all castrated goats from 4 hours post-castration, reaching peak concentration at 20 hours post-castration with the highest recorded in goats castrated by orchidectomy (63.34 ± 1.49 pg/mL; 120.44 ± 3.74 pg/mL), followed by in-situ spermatic cord ligation (25.08 ± 2.19 pg/mL; 109.77 ± 2.97 pg/mL) and then Burdizzo (61.36 ± 2.63 pg/mL; 87.29 ± 3.92 pg/mL). This was followed by a significant (p=0.041) decrease by 24 hours post-castration and non-significant (p=0.101) fluctuations up to 72 hours post-castration in all castrated bucks. In conclusion, all the castration methods induced changes in serum concentration of SAA, which was less marked in Burdizzo and in-situ spermatic cord ligation compared to orchidectomy.