Application of Competitive Elisa (Compelisa) Rose Bengal Plate Test (RBPT) and Serum Agglutination Test (SAT) for Detection of Antibodies to Brucella Infection in Slaughter Cattle in Sokoto, Nigeria

Abstract

The application of Rose Bengal Plate Test, serum agglutination and competitive Elisa was employed for the detection of Brucella antibodies in slaughtered cattle in Sokoto metropolis. It was observed that of the 1711 screened serum samples, 383 (22.38%) 376 (21.97%) and 395 (23.08%) were positive for Brucella using RBPT, SAT and competitive Elisa, respectively. The females had a higher prevalence than the males in each of the three tests (23.63) RBPT, (23.05%) SAT and (24.49) Competitive Elisa. On age, the highest prevalence was recorded within the age of 24 months and above for all the three tests. The breed with the highest prevalence was Arzuwaq. Of the 3 seasons, dry season recorded the highest prevalence. Based on these results, it was observed that application of more than one test method might ensure more sensitivity and specificity of the diagnosis of brucellosis.

Keywords: Application, SAT, RBPT Compelisa, slaughter cattle

Sahel Journal of Veterinary Sciences Vol. 5 (1) 2006 pp. 9-12