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Standardisation of the nitro-blue tetrazohm test and factors affecting its clinical application


R. Sher
R. Anderson
A.R. Rabson
H.J. Koornhof

Abstract

Attempting to standardise the nitro-blue tetrazolium (NBT) test, the following modifications of previously described procedures were introduced. bucoeytsrich plasma obtained by simple gravitation at 37OC was mixed with NBT reagent which had previously been centrifuged to remove undissolved particles. Thick swan were made and stained with dilute haematoxylin, and physical manipulation during the various procedures was reduced to a minimum. Reconstitution of the buffy coat in fresh normal serum did not alter the NBT result. ormal NET readings were obtained in pregnant females, and women on contraceptive hormonal preparations.

S. Afr. Med. J., 48, 209 (1974).

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eISSN: 2078-5135
print ISSN: 0256-9574