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Investigation into the Aroma of Rosemary using Multi-Channel Silicone Rubber Traps, Off-line Olfactometry and Comprehensive Two-dimensional Gas Chromatography-Mass Spectrometry
Abstract
Multi-channel polydimethylsiloxane rubber traps were used to sample the headspace of rosemary samples (two essential oils from different sources, one oleoresin and one dried herb) followed by comprehensive two-dimensional gas chromatography – time of flight mass spectrometry(GCxGC-TOFMS)orGC-MSanalyses. The aromaof different headspace samples was characterized using a custom-built olfactory apparatus. The differences between the aroma profiles were evident from bubble plots of the perceived aroma at different temperatures. The samples were heat-treated to simulate cooking of food products, and were then reassessed to determine any changes in the aroma profile. It was found that the intense menthol and cooling aromas subsided in all the samples with heating. GCxGC-TOFMS allowed for separation of the numerous components in the headspace samples. Manyterpenes and aliphatics were thus tentatively identified and the relative peak areas were compared to better understand the mixture that contributes to the rosemary aroma.
KEYWORDS Rosemary, multi-channel silicone rubber traps, GCxGC-TOFMS, essential oil, off-line olfactometry.
