Effect of conjugated linoleic acid (CLA) on the performance and serum variables of broiler chickens intoxicated with aflatoxin B1

Abstract

The purpose of this study was to investigate the protective effect of dietary conjugated linoleic acid (CLA) on the prevention of aflatoxin B₁) (AFB₁) toxicity in the broiler chicken. A total of 99 Ross 308 male broiler chicks was assigned to nine treatments for 42 d. The experiment had a 3 X 3 factorial arrangement of treatments involving 0, 2 and 4 g CLA/kg feed, and 0, 200 and 300 ng AFB₁) /kg feed. The parameters evaluated, were feed intake, body weight gain, feed efficiency, mortality, relative weights of liver and serum levels of total protein, total albumin, uric acid, alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Aflatoxin B₁) in the diet negatively affected body weight gain and feed efficiency parameters when the birds were 21 and 42 days of age. Altered serum biochemical or hematologic measurements induced by AFB₁) treatments included increased serum activities of ALP, AST and ALT, decreased serum concentrations of albumin, total protein and uric acid. Liver weight was increased in birds given AFB₁) contaminated diet. The results indicated that CLA alone had insignificant effects on all parameters tested. However, birds receiving CLA+AFB₁) had a significantly higher body weight gain on day 42 than groups receiving AFB₁) alone. In addition, co-treatment with AFB₁) and CLA resulted in a significant improvement in feed efficiency and decreased relative weight of liver as compared with the AFB₁) groups. These results suggest that CLA provided protection against negative effects on liver damage induced by AFB₁) in broiler chickens.


South African Journal of Animal Science Vol.34(2) 2004: 97-103