Relationship between sperm plasma membrane integrity and morphology and fertility following artificial insemination

Abstract

Sperm quality plays an important role in determining fertility. The aim of the study was to examine the relationship between sperm plasma membrane integrity and morphology, and fertility following artificial insemination (AI). A total of 16 ejaculates were collected from three Large White boars using the gloved hand technique. The semen was extended with a commercial extender. The AI dose contained 80 mL semen sample (3 × 10⁹ sperm/mL). Aliquots of diluted semen were assessed for sperm plasma membrane integrity (synthetic binding CD-14 (SYBR+)/propidium iodide (PI-) and sperm morphology (eosin nigrosin). A total of 73 Duroc-type, Large White and nondescript multiparous sows from smallholder farms were inseminated with extended semen samples. Boar sperm plasma membrane integrity and morphology were subjected to one-way analysis of variance (ANOVA). The average boar sperm plasma membrane integrity and normal sperm morphology were 78.6% and 77.2%, respectively. The average conception and farrowing rates following artificial insemination (AI) were 78.1 and 57.5%, respectively. A negative correlation was observed between sperm plasma membrane integrity and fertility. There was a weak positive correlation between normal sperm morphology and conception rate (r = 0.11). Additionally, a relationship was observed between normal sperm morphology and litter size (r = 0.37) and total number born alive (r = 0.03), although relatively low. In conclusion, a negative relationship was found between sperm plasma membrane integrity and fertility. Moreover, there was a relationship between morphologically normal sperm and litter size, as well as number of piglets born alive, although relatively low.

Keywords: Boar, Eosin Nigrosin, Semen quality, SYBR14/PI