Evidence for a functional role of the pineal in bovines

Abstract

Dat die epifise moontlik n regulerende invloed op die gonadotrofiese funksie van die adenohipofise ultoefen, is m.b.v. in vitro tegnieke ondersoek. Bykomstig, is n aaneenlopende stelsel ontwerp om die meganisme van die werking van die antionadotrofiese beginsel van die epifise te bestudeer, aangesien daar n direkte negatiewe terugvoer van hiposifiele LH op die hipothalamus voorgekom het toe adenohipofisiale en hipothalamiese weefsel gesamentlik gekweek is. Resultate het aangetoon dat die hormone van die epifise die vrystelling van die tersake hipothalamiese vrystellingsfaktore inhibeer, waardeur die afskeiding van LH deur die adenohipofise verminder word. Laastens toon die resultate aan dat die epifise die produksie van LH-inhiberende faktore geproduseer deur die hipothalamus, stimuleer. 

SUMMARY:

The possibility that the pineal exerts a regulatory influence on the gonadotrophic function of the adenohypophysis was investigated using in vitro techniques. Additionally, since a direct negative feedback of pituitary LH onto the hypothalamus occured when adenohypophysical and hypothalamic tiisisues were incubated together, a continuous-flow system was devised to study the mechanisms of action of the pineal anti-gonadotrophic principles. Results demonstrated that the bovine pineal has marked anti-gonadotrophic properties in vitro. Data showed that the pineal hormones inhibited the release of the relevant hypothalamic release factor, thereby reducing secretion of LH from the adenohypophysis. Finally, results indicated that the pineal also stimulated the production by the hypothalamus of an LH-inhibitory factor.