Development of a radioimmunoassay to measure luteinizing hormone in bovine blood

Abstract

A radioimmunoassay using tubes coated with antibody was developed to measure luteinizing hormone (LH) in bovine blood. Antisera to bovine LH (NIH-LH-B7) were raised in rabbits. Non-specificity of pooled antisera was removed from the antisera by adsorption. The reliability of the assay for use in physiological studies is described.