Extraction and classification of lipids from seeds of persea Americana miller and chrysophyllum albidum g. Don.

Abstract

Seed lipids of Persea americana and Chrysophyllum albidum were studied. Lipids were extracted with chloroform-methanol, analysed with silicic column chromatography and thin
layer chromatography. The total lipid content of Persea americana was 10.8% while that of Chrysophyllum albidum was 7.7%. Fractionation of the total lipid fraction of Persea americana using silicic acid column chromatography showed the occurrence of neutral lipids 6.5%, glycolipids 2.1% and phospholipids 2.2% while those of Chrysophyllum albidum were
4.2%, 1.7% and 1.8% for neutral lipids, gycolipids and phospholipids, respectively. Thin layer chromatography separation showed three classes of lipids in Persea americana and Chrysophyllum albidum which were oleic acid, palmitic acid and cholesterol. The colour of these lipids was yellow and solid at room temperature. These studies suggest that oil extracted from seeds of Persea americana and Chrysophyllum albidum could be used for the manufacture of industrial products.