Acute anti-stress properties of polyherbal formulated tea (Citrus limon, Curcuma longa, Zingiber officinale, Allium sativum, and Moringa oleifera)

Abstract

Polyherbal formulation involves combining multiple herbs in specific ratios to create potent mixtures for the treatment of illnesses. The aim of this study is to evaluate the acute anti-stress activity of poly-herbal-formulated teas (Curcuma longa, Moringa oleifera, Zingiber officinale Roscoe, Citrus limon, Allium sativum, and Syzygium aromaticum). This study was evaluated using a tail suspension, a force swimming test, and a cold restraint stress test. The stress control group had more mobility during the tail suspension test than the groups that were given the polyherbal-formulated tea at 5 mg/kg, 10 mg/kg, and Standard drug group, fluoxetine, 20 mg/kg (p <0.05). In the forced swimming test, the polyherbal-formulated tea at 10 mg/kg and fluoxetine at 20 mg/kg increased swimming time when compared with stress control (p <0.05). In the cold restraint test, the polyherbal-formulated tea at 5 mg/kg, 10 mg/kg, and fluoxetine at 20 mg/kg reduced the cortisol level in mice when compared to stress control (p <0.05). When compared to the stress control, the polyherbal tea at 5 mg/kg and 10 mg/kg significantly raised the activity of superoxide dismutase in mice (p <0.001). The activity of catalase went up when polyherbal-formulated tea at 10 mg/kg and fluoxetine (20 mg/kg) were given compared to stress control. The levels of malondialdehyde (MDA)decreasewhen the animals were administered5 mg/kg and 10 mg/kg, compared to the stress control group (p<0.05). In conclusion, the polyherbal-formulated tea possesses antistress properties.