Effect of ethanol extract of the fruiting bodies of organically cultivated Pleurotusostreatus on the haematological indices of high sucrose- high fat diet-streptozotocin induced diabetic rats

Abstract

The effect of ethanol extract of the fruiting bodies of organically cultivated Pleurotusostreatus on the haematologicalindicesof HS-HFD-Streptozotocin induced diabetic rats was determined using standard methods. The pharmacological model was 20% High Sucrose (HS) + 20% High Fat Diet (HFD) + 35mg/kg body weight (via intraperitoneal) Streptozotocin (STZ) induced diabetic rat model, with the fruiting body ethanol extracts administered orally at 50, 150 and 300mg/kgb.w.The level of RBC indices, (count, Hb, HCT, MCH, MCHC and MCV in all the groups treated with ethanol extracts of P.ostreatus, the reference group and the control group were significantly(p< 0.05) higher compared to the diabetic control group while the WBC,PLT, MPV, PDW , PCTRDW-CV and RDW-SD levels in the P.ostreatus ethanol extract groups, metformin reference treatment group and the normal control were significantly ((p< 0.05) lower compared to the diabetic control groupafter the 3rd, 6th and 9th week of treatment respectively.The results of the study revealed that the RBC , hemoglobin, HCT, MCH, MCHC and MCV levels were significantly raised within normal ranges by the extract in a time and dose dependent manner in the treated diabetic rats. The extracts also showed positive effects on WBC, PLT, MPV, PDW, PCT, RDW-CV and RDW-SD levels by lowering them towards the normal ranges, time and dose dependently. These results therefore suggest that ethanol extract of the fruiting bodies of organically cultivated Pleurotusostreatus may be employed in the management of anaemia, prevent bleeding or disorder of platelet function and improve immune function in diabetics.