Antimcrobial activity of extract of Bryophyllum pinnatum and Citrus aurantifoliaon some selected pathogens.

Abstract

The inhibitory properties of Bryophyllum pinnatum and lime juice on some selected microorganisms (Staphylococcus aureus,Streptococcus pyogenes, Escherichia coli, Klebsiella pneumoniae, Aspergillus niger and Candida albicans) were investigated. The various concentrations of the extracts were prepared by double dilution method while the ability of the various extracts to inhibit the growth of the pathogenic organisms was determined using the agar well technique. The highest in-vitro antimicrobial activity of methanolic extract of Bryophyllum pinnatum was (13.0+2.83a mm at 200 mg/ml) against Klebsiella pneumoniae while the least antimicrobial activity was (10.5+2.12b and 10.0+0.00e mm at 200 mg/ml) against Streptococcus pyogenes and Candida albicans respectively. The lime juice extracts had the highest in-vitro antimicrobial activity was (11.0+0.00b mm at 200 mg/ml) against Staphylococcus aureus while the least antimicrobial activity was (7.0+ 0.0b
mm at 200 mg/ml) against Streptococcus pyogenes. The methanol and aqueous soluble plant extracts were active against all the test isolates but methanol extract of Bryophyllum pinnatum demonstrated greater inhibitory activity (MIC) on Staphylococcus aureus, Escherichia coli, Streptococcus species and Aspergillus niger at the range of 12.5 mg/ml. The lime juice extracts demonstrated greater inhibitory activity (MIC) on Escherichia coli at 12.5 mg/ml. The results in the findings showed that the methanolic and aqueous extracts of Bryophyllum pinnatum as well as lime juice extracts have broad-spectrum antimicrobial activity and can serve as natural therapeutic agent against some enteric pathogens.