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Published:
Jan 8, 2013DOI:
10.4314/rj.v28i1.6Keywords:
Banana tissue culture sugars table sugar laboratory grade sucrose mannitol
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Comparative study of effects of table sugar, laboratory grade sucrose and mannitol on growth of banana plantlets under in vitro conditions
Abstract
A study was conducted to investigate effects of table sugar, laboratory grade sucrose and mannitol on growth of banana plantlets under in vitro conditions. Plantlets of banana of Injogo variety were grown on Murashige and Skoog (premixed) basal
medium with vitamins (4.4g x l-1) supplemented with 1ìM benzylaminopurine and 1ìM indole acetic acid. The experiment was executed in two phases. In the first phase, 30g x l-1 of laboratory grade sucrose, table sucrose and mannitol was used with no sugar as a control treatment. In the second phase of the experiment, the quantity of carbohydrates was reduced to 15g x l-1. Data on growth rate and fresh weigh gain was collected. After four weeks, plants grown on the culture medium with laboratory grade sucrose showed consistently higher growth rate and more gain in fresh weight (2.395 and 1.039g
respectively for 30 and 15g of sugar per litre of culture medium) as compared to the other treatments (1.397, 0.110 and 0.373g for 30g of sugar per litre of culture medium, and 0.767, 0.355 and 0.638g for 15g of sugar per litre of culture medium, respectively for the table sugar, mannitol and control). The plantlets grown on the culture medium with mannitol showed the lowest gain of fresh weight. This indicates that mannitol is a
poor source on energy for banana plantlets. This study indicated a possibility of replacing the laboratory grade sucrose by the table sugar without significant loss in quality and growth so as to reduce the production cost of in vitro plantlets of banana.
medium with vitamins (4.4g x l-1) supplemented with 1ìM benzylaminopurine and 1ìM indole acetic acid. The experiment was executed in two phases. In the first phase, 30g x l-1 of laboratory grade sucrose, table sucrose and mannitol was used with no sugar as a control treatment. In the second phase of the experiment, the quantity of carbohydrates was reduced to 15g x l-1. Data on growth rate and fresh weigh gain was collected. After four weeks, plants grown on the culture medium with laboratory grade sucrose showed consistently higher growth rate and more gain in fresh weight (2.395 and 1.039g
respectively for 30 and 15g of sugar per litre of culture medium) as compared to the other treatments (1.397, 0.110 and 0.373g for 30g of sugar per litre of culture medium, and 0.767, 0.355 and 0.638g for 15g of sugar per litre of culture medium, respectively for the table sugar, mannitol and control). The plantlets grown on the culture medium with mannitol showed the lowest gain of fresh weight. This indicates that mannitol is a
poor source on energy for banana plantlets. This study indicated a possibility of replacing the laboratory grade sucrose by the table sugar without significant loss in quality and growth so as to reduce the production cost of in vitro plantlets of banana.
