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Published:
Oct 9, 2020DOI:
10.4314/rejhs.v8i3.1Keywords:
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Main Article Content
Exogenous Calcium Fibrin Sealant Loaded with Ageratum conyzoides Linn. Bactericidal Plant Extract: Equilibrium between a Biochemical Activator and Phytochemicals Inhibitors of Thrombin
Abstract
Objective: It is of primary importance to develop wound healing sealant that prevent bacteria contamination and growth. We propose to formulate poor platelets plasma material supplemented with a bactericidal plant extract, Ageratum conyzoides Linn. (Asteraceae). Aqueous extract of this plant is used as a bactericide.
Methodology: Platelet-poor plasma (PPP) containing less than 10,000 platelets per μL were used for all experimentations. Physiological serum (NaCl 0.9%) had served to prepare calcium chloride (CaCl ) solutions at 2 2M, 4M, 6M, 8M and 10M. Clauss fibrinogen assays were done to determine fibrinogen concentrations in each plasma pocket. Clotting times were measured following the addition of the appropriate calcium chloride concentration to the plasma. Plant extract at a concentration of 250 mg/mL in physiological serum was also added to the plasma and followed by the clotting times measured.
Results: Clauss fibrinogen assays reflected a global satisfactory fibrinogen concentration. Overall profile of the evolution of clotting time vs calcium concentrations adopts a curve form and the shortest clotting times are those obtained with 4M calcium concentration. However, the presence of extract deeply disturbed clotting process, as clotting times were extremely elongated.
Conclusion: Our objective was difficult to achieve because of the thrombin inhibitory side effects of the extract which were added to antithrombin III inhibitory effects in presence of excess calcium.
Keywords: Wound healing, fibrin sealant, Ageratum conyzoides Linn., biomaterial, inhibitors.
French title: Formation d'un Biomatériau de Fibrine par Apport Exogène de Calcium et Supplémentation par un Extrait Bactéricide de la plante
Ageratum conyzoides Linn. : Equilibre entre un Activateur Biochimique et des Inhibiteurs Phytochimiques de la Thrombine
Objectif: Il est d'une importance capitale de développer des pansements cicatrisants qui empêchent la contamination et la prolifération bactérienne. Nous nous proposons de formuler un biomatériau cicatrisant de fibrine à base de plasma pauvres en plaquettes et supplémenté d'un extrait aqueux de Ageratum conyzoides Linn.
Méthodes: Du plasma pauvre en plaquettes contenant moins de 10 000 plaquettes par μL a été utilisé pour toutes les expérimentations. Le sérum physiologique (NaCl 0,9%) a servi à la préparation des différentes solutions de calcium aux concentrations de 2M, 4M, 6M, 8M et 10M. Le dosage du fibrinogène par les tests de Clauss a été effectué pour chaque poche de plasma. Les temps de gel ont été déterminés suite à l'addition du calcium à la concentration adéquate. L'extrait de plante à la concentration de 250 mg/mL dans du sérum physiologique a également été additionné au plasma suivi de la détermination des temps de gel.
Résultats: Les tests de Clauss mènent à des taux de fibrinogène moyens satisfaisants. Le profil global de l'évolution des temp de gel en fonction de la concentration en calcium adopte une forme hyperbolique et le temps le plus court est obtenu à 4M de calcium. La présence de l'extrait perturbe profondément la formation du gel puisque les temps de gel de ces derniers sont extrêmement rallongés.
Conclusion: Notre objectif a été difficile à atteindre à cause des effets secondaires inhibiteurs de l'extrait qui se sont ajoutés à ceux de l'antithrombin III en présence d'un excès de calcium.
Mots clé: Cicatrisation ; Pansement fibrine ; Ageratum conyzoides Linn. ; Activateur; Inhibiteurs.
