Evaluation of antibiogram of bacteria isolated from laboratories and lecture theatres in Bayero University Kano

Abstract

This study was aimed to evaluate antibiogram of bacteria within 25 laboratories and 16 lecture theatres in Bayero University, Kano (Old and New sites). Samples of air were randomly collected from the two sites. Observation schedule and questionnaires were used to assess sanitary conditions in the sampling areas. Enumeration, isolation and identification of isolate were carried out using settle plate technique. Meteorological parameters of the sampling sites were also recorded. An antimicrobial susceptibility test isolates against some commercially available disinfectants was also carried out using disc diffusion assay. Based on cultural, morphological and biochemical characteristics, 11 bacterial species were isolated and identified Staphylococcus aureus have the highest frequency of occurrence and Entrobacter earogens, Bacillus sublilis have the lowest frequency of occurrence. Temperature in the laboratories ranged from 27 oC to 33.5 oC while in the lecture theatres ranged from 28.2oC to 33.2oC. Carbon dioxide concentration in the laboratories ranged from 92 ppm to 715 ppm while lecture theatres ranged from 405 ppm and 43 ppm. Relative Humidity in the laboratories ranged from 20 % to 38% while in the lecture theatres ranged from 17% to 38% .The susceptibility of disinfectant on isolate show that Enterobacter aerogens had the highest zone of inhibition of 17 mm on hypo and Pseudomonas aeruginosa have the lowest zone of inhibition of 7mm while sensitivity to dettol show that Staphylococcus epidermidis and Streptococcus pyogenes have the highest zone of inhibition of 15mm each and Enterobacter aerogens have the lowest zone of 10mm, both at 100% concentration, fresh lemon show no zone of inhibition against all the isolates. All the three disinfectants showed no efficacy at concentrations lower than 25%. It is also important to understand that bacteria within public areas has important roles in spreading pneumonia infection and other respiratory diseases.