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Bacterial species isolated from water bodies in Rivers and Bayelsa States are possible aetiological agents of diarrhoea
Abstract
Background: The role of water in the transmission of diarrhoea has been established. The World Health Organization (WHO) reported that diarrhoea disease were among the 10 major causes of death in young children in 11 African, Asia and South American countries.
Aim: To investigate the incidence of Salmonella and Vibrio species as aetiological agents of diarrhoea in water bodies in Rivers and Bayelsa States.
Methods: A total of twenty water samples were collected from rivers and cultured for the various microorganisms.The total heterotrophic counts were determined by Standard Plate Count (SPC) method. Salmonella and Vibrio species were enumerated by an enrichment procedure in Selenite F broth and Alkaline Peptone water (APW) respectively; cultured on Bismuth sulfite agar (BSA) and Thiosulphate citrate bile salt sucrose (TCBS) agar selective for salmonella and vibrio species respectively. The total coliform counts was determined and the Most Probable Number (MPN) using the multiple tube fermentation technique.
Results: The study revealed isolates with various percentage occurrences which include Vibrio (12.1%) and Salmonella (33.3%). Among the coliforms were Escherichia coli (33.3%), Klebsiella (22.2%), Enterobacter (33.3%) and Citrobacter (11.1%).MPN values for enumeration of coliforms ranged from 80/100 ml to = 16,000/100 ml of the water samples analysed. Total heterotrophic count per milliliter of the original water sample ranged between 1.0 × 106 and 3.0 ×108 cfu/ml.
Conclusion: The water sources examined have proved to be potential sources of diarrhoea organisms and also could lead to disease outbreak because of the significant number of these organisms in these water bodies.
Aim: To investigate the incidence of Salmonella and Vibrio species as aetiological agents of diarrhoea in water bodies in Rivers and Bayelsa States.
Methods: A total of twenty water samples were collected from rivers and cultured for the various microorganisms.The total heterotrophic counts were determined by Standard Plate Count (SPC) method. Salmonella and Vibrio species were enumerated by an enrichment procedure in Selenite F broth and Alkaline Peptone water (APW) respectively; cultured on Bismuth sulfite agar (BSA) and Thiosulphate citrate bile salt sucrose (TCBS) agar selective for salmonella and vibrio species respectively. The total coliform counts was determined and the Most Probable Number (MPN) using the multiple tube fermentation technique.
Results: The study revealed isolates with various percentage occurrences which include Vibrio (12.1%) and Salmonella (33.3%). Among the coliforms were Escherichia coli (33.3%), Klebsiella (22.2%), Enterobacter (33.3%) and Citrobacter (11.1%).MPN values for enumeration of coliforms ranged from 80/100 ml to = 16,000/100 ml of the water samples analysed. Total heterotrophic count per milliliter of the original water sample ranged between 1.0 × 106 and 3.0 ×108 cfu/ml.
Conclusion: The water sources examined have proved to be potential sources of diarrhoea organisms and also could lead to disease outbreak because of the significant number of these organisms in these water bodies.
