Loveline Ngu
Laboratory of Vaccinology/Biobanking, Chantal Biya International Reference Center for Research on the Prevention and Management of HIV/AIDS, Yaounde, Cameroon; Department of Biochmistry, Faculty of Sciences, University of Yaounde I, Cameroon
Herve Fotso Ouambo
Laboratory of Vaccinology/Biobanking, Chantal Biya International Reference Center for Research on the Prevention and Management of HIV/AIDS, Yaounde, Cameroon; Pan African Center for Clinical and Translational Sciences (PANECTS), Yaounde, Cameroon
Inès Nyebe
Laboratory of Vaccinology/Biobanking, Chantal Biya International Reference Center for Research on the Prevention and Management of HIV/AIDS, Yaounde, Cameroon; Pan African Center for Clinical and Translational Sciences (PANECTS), Yaounde, Cameroon; Department of Microbiology, Faculty of Sciences, University of Yaounde I, Yaounde, Cameroon
Jules Colince Tchadji
Laboratory of Vaccinology/Biobanking, Chantal Biya International Reference Center for Research on the Prevention and Management of HIV/AIDS, Yaounde, Cameroon; Department of Animal Biology and Physiology, Faculty Of Sciences, University of Yaounde I, Cameroon
Georgia Ambada
Laboratory of Vaccinology/Biobanking, Chantal Biya International Reference Center for Research on the Prevention and Management of HIV/AIDS, Yaounde, Cameroon; Department of Animal Biology and Physiology, Faculty Of Sciences, University of Yaounde I, Cameroon
Akelekeh Ndah
Pan African Center for Clinical and Translational Sciences (PANECTS), Yaounde, Cameroon
Bloomfield Atechi
Pan African Center for Clinical and Translational Sciences (PANECTS), Yaounde, Cameroon
Abel Lissom
Laboratory of Vaccinology/Biobanking, Chantal Biya International Reference Center for Research on the Prevention and Management of HIV/AIDS, Yaounde, Cameroon; Department of Biological Sciences, Faculty of Sciences, University of Bamenda, Bamenda, Cameroon
Philémon Etienne Atabonkeng
Department of Biochemistry, University of Dschang, Dschang, Cameroon
George Chukwuma
Department of Medical Laboratory Science, College of Health Sciences, Nnamdi Azikiwe University, Awka, Nigeria
Vitalis Efezeuh
Department of Biochemistry and Molecular Biology, University of Buea, Buea, Cameroon
Park Chae Gyu
Laboratory of Immunology, Brain Korea 21 PLUS Project for Medical Science, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, Republic of Korea
Charles Esimone
Department of Pharmaceutical Microbiology and Biotechnology, Nnamdi Azikiwe University, Awka, Nigeria
Jules Clement Nguedia Assob
Department of Medical Laboratory Sciences, University of Buea, Buea, Cameroon
Eric Akum Achidi
Department of Medical Laboratory Sciences, University of Buea, Buea, Cameroon
Malachy Okeke
Department of Natural and Environmental Sciences, Biomedical Science Concentration, School of Arts and Sciences, American University of Nigeria, 98 Lamido Zubairu Way, Yola, Nigeria
Vincent Pryde Kehdingha Titanji
Biotechnology Units, Department of Biochemistry and Molecular Biology, University of Buea, Buea, Cameroon
Wilfred Mbacham
Department of Biochmistry, Faculty of Sciences, University of Yaounde I, Cameroon
Alain Bopda-Waffo
Pan African Center for Clinical and Translational Sciences (PANECTS), Yaounde, Cameroon; Biochemistry and Molecular Biology, Indiana University School of Medicine, 635 Barnhill Drive, MS1017Q Lab MS1015, Indianapolis, IN, United States of America
Godwin Wapimewah Nchinda
Laboratory of Vaccinology/Biobanking, Chantal Biya International Reference Center for Research on the Prevention and Management of HIV/AIDS, Yaounde, Cameroon; Pan African Center for Clinical and Translational Sciences (PANECTS), Yaounde, Cameroon; Department of Pharmaceutical Microbiology and Biotechnology, Nnamdi Azikiwe University, Awka, Nigeria
Abstract
Introduction: in areas with intense perennial malaria transmission, limited data is available on the impact of environmental conditions especially rainfall on naturally acquired immunity against promising malaria vaccine candidates. For this reason, we have compared IgG antibody responses specific to Plasmodium spp. derived MSP3 and UB05 vaccine candidates, in plasma of children living in two areas of Cameroon differing in rainfall conditions.
Methods: data about children less than 5 years old was collected during the years 2017 and 2018. Next malaria asymptomatic P. falciparum (Pf) infected children were selected following malaria test confirmation. MSP3 and UB05 specific IgG antibody responses were measured in participant´s plasma using enzyme-linked immunosorbent assay (ELISA).
Results: interestingly, IgG antibody responses specific to UB05 were significantly higher (p<0.0001) in Pf-negative children when compared to their asymptomatic Pf-infected counterparts living in monomodal rainfall area. In contrast, a significantly higher (p<0.0001) IgG response to MSP3 was observed instead in asymptomatic Pf-infected children in the same population. In addition, IgG responses specific to UB05 remained significantly higher in bimodal when compared to monomodal rainfall areas irrespective of children´s Pf infection status (p<0.0055 for Pf-positive and p<0.0001 for negative children). On the contrary, IgG antibody responses specific to MSP3 were significantly higher in bimodal relative to monomodal rainfall areas (P<0.0001) just for Pf-negative children.
Conclusion: thus IgG antibody responses specific to UBO5 are a better correlate of naturally acquired immunity against malaria in Pf-negative Cameroonian children especially in monomodal rainfall areas.
