Pan African Medical Journal
Journal / Pan African Medical Journal / Vol. 45 No. 1 (2023) / Articles

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Published:

Apr 8, 2024

Keywords:

Methicillin-resistant Staphylococcus aureus wounds nasopharynx urine Buea Health District

Issue

Vol. 45 No. 1 (2023)

Section

Articles

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Morgan Mokeo Ndedy

Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon; Laboratory for Emerging Infectious Diseases, University of Buea, Buea, South West Region, Cameroon

Raymond Babila Nyasa

Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon

Seraphine Nkie Esemu

Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon; Laboratory for Emerging Infectious Diseases, University of Buea, Buea, South West Region, Cameroon

Jerome Achah Kfusi

Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon; Laboratory for Emerging Infectious Diseases, University of Buea, Buea, South West Region, Cameroon

Nene Kaah Keneh

Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon; Laboratory for Emerging Infectious Diseases, University of Buea, Buea, South West Region, Cameroon

Thomas Njinuwoh Masalla

Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon; Laboratory for Emerging Infectious Diseases, University of Buea, Buea, South West Region, Cameroon

Lucy Mande Ndip

Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon; Laboratory for Emerging Infectious Diseases, University of Buea, Buea, South West Region, Cameroon

Abstract

Introduction: Staphylococcus aureus, which is part of the normal flora accounts for most acute and chronic infections in humans, and treatment options are greatly limited, when infection is caused by methicillin-resistant Staphylococcus aureus (MRSA). This study was to determine the prevalence and antimicrobial susceptibility pattern of MRSA from clinical samples obtained randomly from patients in Buea Health District.

Methods: a total of 264 wounds, nasopharynx, and urine samples were collected from patients from different hospitals in Buea and transported to the laboratory in the University of Buea, for analysis. Samples were inoculated on mannitol salt agar for S. aureus isolation, characterized morphologically by gram staining and biochemically by catalase, coagulase, and hemolysis tests. Diagnosis of S. aureus was confirmed by molecular identification of the nuc gene. MRSA was identified from S. aureus by oxacillin screening and confirmed by molecular identification of the mecA gene. The data were analyzed using SPSS version 17.0.

Results: S. aureus was isolated from 70 (26.52%) and all were confirmed molecularly by nuc gene amplification. MRSA by oxacillin screening was 36 (13.64%) while MRSA detected by mecA gene amplification was 34 (12.88%). Antimicrobial susceptibility testing revealed 100% resistance to ampicillin, 88.24% to cefixime and 70.59% to ceftriaxone while low resistance was observed to meropenem (11.76%), doxycycline (14.71%), and vancomycin (17.67%).

Conclusion: MRSA isolated from Buea Health District are resistant to ampicillin, cefixime, and ceftriaxone. The antimicrobials (meropenem, doxycycline, and vancomycin) should be used to treat MRSA infections in Buea Health District.

Journal Identifiers

eISSN: 1937-8688