Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hosts

Abstract

Background: Individual host susceptibility is believed to be a risk factor in the interaction between the host and the
parasite. Since studying time series in humans is limited, animal models are replaced.

Aim: This study aims to explore and compare the pattern of inflammatory cell types along the biliary tract and their  association with proliferative lesions in the early development of cholangiocarcinoma from susceptible and nonsusceptible animal models.

Methods: Thirty male Syrian golden hamsters and 30 BALB/c mice, serving as the susceptible and nonsusceptible  animal models, were used in this comparative study. The animals were infected with 50 Opisthorchis viverrini  metacercariae via gastric intubation. At days 1, 2, 7, 14, 28, and 56 postinfection (p.i.), five animals were randomly  selected from each group and humanely sacrificed. The hepatobiliary tissues were collected and processed for  histopathological study. Histochemical and immunohistochemical staining were applied to differentiate the  inflammatory cell types. Kruskal– Wallis and Mann–Whitney tests were applied to assess all semi-quantitative and  quantitative variables. The correlation between each variable was also analyzed using Spearman rank at a p-value < 0.05.

Results: The results demonstrated that mice had different patterns of infiltrating cell types when compared to  hamsters. This suggested that the cellular response to the infection in mice occurred earlier than that in hamsters. The  response in mice reached its peak at D7 to D14 and then rapidly declined at D28. In contrast, although the inflammatory  response in hamsters started slowly, the response reached the peak at D28 and maintained a high level until D56.  Significant differences in the number of inflammatory cells between mice and hamsters were seen at D1 (p = 0.047), D7  (p = 0.049), D28 (p = 0.040), and D56 (p < 0.040).

Conclusion: The inflammatory responses to O. viverrini infection in the  nonsusceptible animal model occurred and declined earlier while the response in the susceptible animal model  occurred later in a gradual manner. Both rodents are suitable animal models for the studies of opisthorchiasis  susceptibility.