Galleria mellonella (greater wax moth) as an eco-friendly in vivo approach for the assessment of the acute toxicity of medicinal plants: Application to some plants from Cameroon

Abstract

Background: The evaluation of medicinal plants’ toxicity is a prerequisite prior to their usage. The vertebrate models  used for this purpose are often the object of ethical consideration. Though invertebrate models including Galleria mellonella (GM) have demonstrated the ability to be used to assess the toxicity of various products. To the authors’ knowledge, GM has never been exploited to determine the toxicity of medicinal plants.
Aim: The aim of this study was to demonstrate the potential of GM larvae as a simple, inexpensive, and rapid model for the evaluation of the toxicity of herbal medicines.
Method: In this study, the toxicity of aqueous and ethanolic (80%, v/v) extracts of seven well known plants from Cameroon namely leaves of Cymbopogon citratus (DC.) Stapf, Moringa oleifera Lam and Vernonia amygdalina Delile; barks of Cinchona officinalis and Enantia chlorantha Oliv; barks and seeds of Garcinia lucida Vesque and leaves and seeds of Azadirachta indica (Neem) was evaluated using the larval form of GM. The median lethal doses (LD₅₀), 90% (LD₉₀), and 100% (LD₁₀₀) were determined using the spline cubic survival curves and equations from the data obtained on the survival rate of GM 24 hours after the injection with the extracts.
Results: We found that distilled water extracted a more important mass of phytochemical compounds (7.4%–21.2%) compared to ethanolic solution (5.8%–12.4%). LD varied depending on the plant materials and ethanolic extracts (hydroalcoholic extract, (HAE)) were more toxic to GM than aqueous ones. The LD₅₀ (mg/ml) of the tested extracts varied from 4.87 [3.90 g/kg body weight (bw)] to >200 (> 166.67 g/kg bw), the LD₉₀ (mg/ml) from 25.00 (18.52 g/kg bw) to >200 (> 181.82 g/kg bw) and LD₁₀₀ (mg/ml) from 45.00 (40.91 g/kg bw) to > 200 (>181.82 g/kg bw). The HAE of A. indica seed and C. officinalis bark exhibited the highest toxicity with LD50 (g/kg bw) of 3.90 and 4.81, respectively.
Conclusion: The results obtained in this study suggest that GM can be used as a sensitive, reliable, and robust ecofriendly model to gauge the toxicity of medicinal plants. Thus, avoid the sacrifice of vertebrate models often used for this purpose to limit ethical concerns.