Impact of Perfusion on Growth and Drug Delivery in Vascularized Tumor Spheroid-On-A-Chip Models

Abstract

Vascularized tumors are central to cancer research, significantly impacting tumor development and medication delivery dynamics. The attainment of precise depictions of these occurrences is essential in developing productive treatment approaches. Current methodologies often need to be revised to replicate authentic tumor microenvironments accurately. The research proposed the Vascularized Tumor Spheroid-on-a-Chip Model (VTSoCM) to tackle these issues. This novel model combines multi-scale modeling and microfluidic technologies to provide a more precise and dynamic representation of vascularized tumors. The VTSoCM exhibits significant progress compared to current methodologies. The microvessel density is notably elevated at a value of 30.12 vessels per square millimeter, suggesting a heightened capacity for angiogenesis. The Drug Penetration Depth has been measured to be 0.78 mm, enabling more accurate drug delivery evaluations. The growth rate of tumor spheroids increases to 4.25 mm³ each day, indicating a more realistic tumor advancement. The migration distance of immune cells has been seen to extend up to 230 μm, facilitating a more comprehensive comprehension of immunological responses. The permeability of the vascular network demonstrates a rise to a value of 0.038 cm/s, providing valuable insights into the process of tumor angiogenesis. The results highlight the capacity of VTSoCM to transform the cancer research field and advance treatment approaches significantly. The model has significant potential to propel the development of tailored cancer therapies, eventually leading to enhanced patient outcomes.