Prevalence study of methicillin resistant Staphylococcus aureus and its SCCmec features in horses and handlers in Zaria and Kaduna, Nigeria

Abstract

Methicillin resistant Staphylococcus aureus (MRSA) is a pathogen with public health implications being resistant to most used antibiotics. It has been associated with health facilities but has now become prevalent in community settings. The presence of MRSA in 240 apparently healthy horses and 65 horse handlers in Kaduna and Zaria was studied through nasal swabs collection by a one-stage cluster sampling and analysed using standard microbiological tests and genotyping methods. Questionnaires were also administered to assess for risk factors associated with MRSA carriage. Prevalence was 10% and 6.2% from the horses and horse handlers respectively. The S. aureus isolates showed highest resistance rates of 54.6% to penicillin and oxacillin. There were high resistance rates also to erythromycin, and tetracycline of 47.7% and 34.1% respectively. Resistance of 22.7% to amikacin and gentamicin, 6.8% to sulphamethoxazole+trimethoprim, 2.3% to ciprofloxacin, and 2.3% to chloramphenicol were also observed. Multidrug resistance (MDR) was found among 74.8% of the MRSA isolates. SCCmec typing showed types I, III, and IV in both the horses and the horse handlers while one horse was found to carry SCCmec V. Possible risk factors identified for MRSA carriage by horse handlers included being a veterinarian, exposure to antimicrobial agents, healthcare facility visitation, and personal hygiene. For the horses, risk factors from this study were allergy and wound management. MRSA nasal carriage in horses and horse handlers, as well as MDR (multidrug resistant) strains of S. aureus have been established from this study, this is of public health concern.