Molecular identification and phylogenetic analysis of acetylcholinesterase-2 gene from Culex pipiens and Culex quinquefasciatus (Diptera: Culicine) in Nigeria

Abstract

West Nile virus (WNV) is maintained in an enzootic cycle between ornithophilic mosquitoes and birds. There is paucity of information on population density of these vectors and the molecular details in Nigeria. We determined relative abundance and employed molecular methods to detect Culex species. Mosquitoes were caught from April 2013 to February 2014. Genomic DNA extraction was carried out and acetylcholinesterase 2 gene amplified by Polymerase Chain Reaction in different Culex species. Culex pipiens quinquefasciatus and Cx. p. pipiens were identified in different locations, while some other Culex species could not be identified. Culex p. quinquefasciatus were sequenced by Sanger’s method. Vector density was 100 Culex species per man hour. Identification of Culex pipiens and Cx. quinquefasciatus from different locations in Ibadan, Nigeria reaffirms the presence of vectors and highlights possibility of enzootic transmission of WNV. Surveillance should be sustained in equine population and bird reservoirs.

Keywords: Culex pipiens; Culex quinquefasciatus; Nigeria