Main Article Content
Catalase activity, lipid peroxidation, cholesterol and triglyceride levels in alloxan – induced diabetes mellitus in female and male rats.
Abstract
Background: Diabetes mellitus is a disorder of
carbohydrate metabolism and is associated with oxidative
reactions.
Objective: The present study is to determine the activities of
catalase, lipid peroxidation, glucose, protein, cholesterol
and triglyceride concentrations in the liver and kidney in
alloxan-induced diabetes in female and male rats. In
addition, the study is to ascertain if gender differences affect
oxidative stress in diabetes.
Methods : Forty male (165±8.46g) and female
(162.7±7.94g) albino Sprague Dawley rats were used for the
study. The rats were injected intraperitoneally with a single
dose of 150mg/body weight of alloxan monohydrate, to
induce diabetes for 14 days. The rats were divided into four
groups, consisting of 10 diabetic male, 10 non-diabetic
male, 10 diabetic female and 10 non-diabetic female. The
rats were fed rat chow and water ad libitum for 14 days and
then sacrificed by decapitation. Blood was taken by cardiac
puncture, while liver and kidney were quickly excised. The
catalase activity, lipid peroxidation, glucose, protein,
cholesterol and triglyceride concentrations in the liver and
kidney of rats were determined.
Results: Rats administered alloxan monohydrate had
elevated plasma glucose levels. The body weights of
diabetic female and male rats were significantly reduced
compared to the non-diabetic rats. The catalase activities in
liver and kidney of diabetic male or female rats were
significantly lower than in non-diabetic rats but the reduction
was more pronounced in diabetic female rats. The liver lipid
peroxidation, cholesterol and triglyceride levels were
significantly higher in the diabetic male or female than in the
non-diabetic rats, but with no significant differences in the
diabetic female or male rats.
Conclusion: Data of the study indicate that sex differences
do not significantly affect oxidative stress in alloxan-induced
diabetes mellitus.
carbohydrate metabolism and is associated with oxidative
reactions.
Objective: The present study is to determine the activities of
catalase, lipid peroxidation, glucose, protein, cholesterol
and triglyceride concentrations in the liver and kidney in
alloxan-induced diabetes in female and male rats. In
addition, the study is to ascertain if gender differences affect
oxidative stress in diabetes.
Methods : Forty male (165±8.46g) and female
(162.7±7.94g) albino Sprague Dawley rats were used for the
study. The rats were injected intraperitoneally with a single
dose of 150mg/body weight of alloxan monohydrate, to
induce diabetes for 14 days. The rats were divided into four
groups, consisting of 10 diabetic male, 10 non-diabetic
male, 10 diabetic female and 10 non-diabetic female. The
rats were fed rat chow and water ad libitum for 14 days and
then sacrificed by decapitation. Blood was taken by cardiac
puncture, while liver and kidney were quickly excised. The
catalase activity, lipid peroxidation, glucose, protein,
cholesterol and triglyceride concentrations in the liver and
kidney of rats were determined.
Results: Rats administered alloxan monohydrate had
elevated plasma glucose levels. The body weights of
diabetic female and male rats were significantly reduced
compared to the non-diabetic rats. The catalase activities in
liver and kidney of diabetic male or female rats were
significantly lower than in non-diabetic rats but the reduction
was more pronounced in diabetic female rats. The liver lipid
peroxidation, cholesterol and triglyceride levels were
significantly higher in the diabetic male or female than in the
non-diabetic rats, but with no significant differences in the
diabetic female or male rats.
Conclusion: Data of the study indicate that sex differences
do not significantly affect oxidative stress in alloxan-induced
diabetes mellitus.
