Biosorption of lead by bacteria isolated from abattoir wastewater

Abstract

Six bacteria were isolated from abattoir wastewater collected from Minna central abattoir. Lead tolerant bacteria were isolated from the wastewater. The isolates were then characterized on the basis of their colonial appearance and reaction to various biochemical tests. The lead tolerance profile of the isolates was carried out using agar diffusion method, with concentrations of Lead nitrate ranging from 50-250 mg/L. Two resistant isolates identified as species of Bacillus and Neisseria were selected for biosorption studies. Lead concentration was determined using Atomic Absorption Spectrophotometry. The lead biosorption capacity of the two isolates was studied by inoculating 2 mL of 24 hours old bacteria suspension in 50mL Nutrient broth, containing varying concentrations of lead (500 and 1000 mg/L) at varying pH (7 and 8), with representative samples being withdrawn at day 4, 8 and 12. The results showed that highest biosorption rate was recorded on day 10, at pH 7, in solution containing 500 mg/L of lead with 75.3% and 66% by Bacillus sp. and Neisseria sp. respectively. These results show that Bacillus sp. had better sorption capacity than Neisseria sp. Both organisms can be used for the removal of lead.

Keywords: Wastewater, Biosorption, Abattoir, heavy metal, Bacteria