In-vitro, anthelminthic effects of Azadirachta indica (Neem), Mallotus philippinensis (Kamala), and Melia azedarach (Bakain) leave extract against Ascaridia galli

Abstract

The present study was conducted to evaluate the anthelmintic activity of aqueous extracts of Azadirachta indica (Neem), Mallotus  philippinensis (Kamala), Melia azedarach (Bakain), and their combination as herbal extracts against Ascaridia galli infection. To determine  efficacy, egg hatch test (EHT) and adult motility assay (AMS) were performed using increasing concentrations of 12.5, 25, 50,  and 100 mg/ml of aqueous plant extracts, in conjunction with 7.5 mg/ml Albendazole as the positive control and phosphate buffered  saline (PBS) as the negative control for up to 15 h. There was 100% mortality achieved with the combined herbal extract (LC₅₀ = 36.7 µg/ml),  whereas mortality with extract from Mallotus philippinensis (LC₅₀ = 41.4 µg/ml), Melia azedarach (LC₅₀ = 147 µg/ml), and  Azadirachta indica (LC₅₀ = 189 µg/ml) were 87.5%, 62.5%, and 50% respectively,  at 15 hours post-exposure. These findings suggest that  Azadirachta indica, Mallotus philippinensis, Melia azedarach herbal extracts, or their combination, should be further exploited to control  Ascaridia galliinfection.