Serological and molecular survey of Trypanosoma evansi in Camels (Camelus dromedarius) in Maiduguri, North-east, Nigeria

Abstract

To date, camels still remain an important work animal as well as source of protein to humans in the Sudan and Sahel regions of Nigeria. Therefore, a cross-sectional study was conducted on 150 camels slaughtered in Maiduguri central abattoir to determine the prevalence of Trypanosoma evansi using Card Agglutination Test (CATT) and Polymerase Chain Reaction (PCR) techniques. Overall, 30 (20%) of the camels tested were seropositive while PCR targeting the 227 base pair of the Variable Surface Glycoprotein (VSG) gene of T. evansi detected the DNA of the parasite in 9 out of the 30seropositive camels. Higher infection was found among adult compared to the young camels using the two diagnostic techniques; 24.1% vs 19.0% and 10.3% vs 4.6%, for CATT and PCR techniques, respectively. However, the differences being not statistically significant (P > 0.05) for the two methods of diagnosis. Furthermore, significantly (P < 0.05)higher prevalence of infection was recorded among male compared to female camels using the serological method of diagnosis, while (P > 0.05) using the molecular method; 27.5% vs 13.6% for CATT and 10.1% vs 2.5% for PCR. Camels with PCV =24 %( mean: 19.8923 ± 4.0931) recorded significantly (P < 0.05) higher prevalence of 23.1% than those with PCV = 25% (mean 31.7294 ± 5.50584), where the prevalence was 17.6%.The results of this study showed that camel trypanosomosis is endemic in the study area.  Furtherstudiesto elucidate the epidemiology and socioeconomic impact of this disease in the northeast region of Nigeria are desirable.

Keywords:Serology, PCR, Dromedary camel, T.evansi, Maiduguri