Morphologic identification and proteomic analysis in adult Haemonchus contortus of West African Dwarf Goats by IDE SDS-PAGE

Abstract

Haemonchus contortus is responsible for most field outbreak of acute and sub-acute parasitic gastro-enteritis of small ruminants. Given the growing prevalence of antihelminthic resistance in parasitic infections there is need for alternative methods of control and the novel option for worm control is vaccines production. This study determines the protein constituents of Haemonchus contortus in both male and female parasites. Two hundred abomasal samples were collected from West African Dwarf Goats at Bodija Municipal Abbattoir, Ibadan, Oyo State, Nigeria. The adult H. contortus were isolated and separated by sex-based on morphological features. Male and female H. contortus were ground separately vortex in Tris buffer and centrifuged at 1000rpm for 5 minutes. In gel separation of the resulting supernatant was achieved using1 dimesnsional Sodium dodecyl sulfate polyacrylamide gel electrophoresis (1DE SDS-PAGE). The raw images of the gel were captured using and analyses on 1D Totallabsoft ware. The software identified 7 (seven) protein bands for male and 10 (ten) protein bands for female and the coefficient of similarities between those bands detected in both male and female parasites was 0.43 (43%). The molecular weight of bands detected for male includes; 145.93,130.91, 64.64, 51.75, 34.68, 20.58,19.35 kDa) while that of female includes (144.62, 102.21, 70.00, 50.28, 35.81, 19.39, 19.49, 19.35, 19.33, 15.98 kDa). This most likely suggests that both male and female parasites have unique antigen of these proteins hitherto present in them. The immunogenicity of the bands can be assessed to identify protein (bands) suitable for vaccines production against haemonchosis in West African Dwarf goat in Nigeria.

Keywords: Haemonchus contortus, parasite sexes, 1DE SDS PAGE, protein bands